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normal human osteoblast cell culture  (Lonza)


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    Lonza normal human osteoblast cell culture
    Normal Human Osteoblast Cell Culture, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+human+osteoblast+cultures/human+normal+primary+osteoblast/pm30054651-13274-12-16
    Average 90 stars, based on 1 article reviews
    normal human osteoblast cell culture - by Bioz Stars, 2026-10
    90/100 stars

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    Cell Culture:

    Article Title: Preclinical validation of Aurora kinases-targeting drugs in osteosarcoma
    Article Snippet: All cell lines were cultured in Iscove's modified Dulbecco's medium (IMDM), supplemented with penicillin (100 U ml −1 )/streptomycin (100 μ g ml −1 ) (Invitrogen Ltd, Paisley, UK) and 10% heat-inactivated fetal bovine serum (FBS; Biowhittaker Europe, Cambrex-Verviers, Belgium). .. Normal human osteoblast cultures (purchased from Lonza Walkersville Inc., Walkersville, MD, USA) were cultured in OGM-osteoblast medium (Lonza Walkersville Inc) for 2–3 weeks before being processed for RNA extraction. ..

    RNA Extraction:

    Article Title: Preclinical validation of Aurora kinases-targeting drugs in osteosarcoma
    Article Snippet: All cell lines were cultured in Iscove's modified Dulbecco's medium (IMDM), supplemented with penicillin (100 U ml −1 )/streptomycin (100 μ g ml −1 ) (Invitrogen Ltd, Paisley, UK) and 10% heat-inactivated fetal bovine serum (FBS; Biowhittaker Europe, Cambrex-Verviers, Belgium). .. Normal human osteoblast cultures (purchased from Lonza Walkersville Inc., Walkersville, MD, USA) were cultured in OGM-osteoblast medium (Lonza Walkersville Inc) for 2–3 weeks before being processed for RNA extraction. ..



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    Figure 7. Effects of hMSC‐ and calvarial osteoblast‐conditioned media on angiogenesis.

    Journal: Tissue Engineering Part A

    Article Title: Effects of Bone Morphogenetic Protein-2 on Neovascularization During Large Bone Defect Regeneration

    doi: 10.1089/ten.tea.2018.0326

    Figure Lengend Snippet: Figure 7. Effects of hMSC‐ and calvarial osteoblast‐conditioned media on angiogenesis.

    Article Snippet: SMAD intensity and localization was quantified using ImageJ.22 Marrow Stromal Cell and Calvarial Osteoblast Culture Human bone marrow stromal cells (hMSCs, ATCC PCS‐500‐012) were purchased from ATCC and cultured according to manufacturer protocols, using ATCC MSC Growth Kit (ATCC PCS‐500‐041).

    Techniques:

    Figure 1 Relative expression of miR-761 in OS tissues and cell lines. (A) miR-761 expression in 61 pairs of OS tissues and corresponding adjacent normal tissues was quantified by RT-qPCR. *P<0.05 vs adjacent normal tissues. (B) RT-qPCR was conducted to analyze miR-761 expression in OS cell lines. A normal human osteoblast hFOB1.19 acts as a control. *P<0.05 vs hFOB1.19. (C) The Kaplan-Meier method was used to determine the effects of miR-761 expression on overall survival in patients with OS.

    Journal: OncoTargets and Therapy

    Article Title:

    MicroRNA-761 targets FGFR1 to suppress the malignancy of osteosarcoma by deactivating PI3K/Akt pathway

    doi: 10.2147/ott.s208263

    Figure Lengend Snippet: Figure 1 Relative expression of miR-761 in OS tissues and cell lines. (A) miR-761 expression in 61 pairs of OS tissues and corresponding adjacent normal tissues was quantified by RT-qPCR. *P<0.05 vs adjacent normal tissues. (B) RT-qPCR was conducted to analyze miR-761 expression in OS cell lines. A normal human osteoblast hFOB1.19 acts as a control. *P<0.05 vs hFOB1.19. (C) The Kaplan-Meier method was used to determine the effects of miR-761 expression on overall survival in patients with OS.

    Article Snippet: Cell culture Normal human osteoblast cells (hFOB1.19) and four human OS cell lines (U2OS, MG-63, HOS and SAOS-2) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Expressing, Quantitative RT-PCR, Control

    Figure 3 FGFR1 is a direct target gene of miR-761 in OS cells. (A) The wild-type and mutant miR-761 binding sites in the 3ʹ-UTR of FGFR1. (B) Agomir-NC or agomir-761 was co-transfected with pmirGLO-WT-FGFR1-3ʹ-UTR and pmirGLO-MUT-FGFR1-3ʹ-UTR into U2OS and HOS cells. After 48 h culture, luciferase reporter assay was carried out to explore whether the 3ʹ-UTR of FGFR1 could be directly targeted by miR-761. *P<0.05 vs agomir-NC. (C, D) The mRNA and protein levels of FGFR1 in U2OS and HOS cells after gain of miR-761 were examined through RT-qPCR and Western blot analysis. *P<0.05 vs agomir-NC. (E) RT-qPCR was utilized to quantify FGFR1 mRNA expression in 61 pairs of OS tissues and corresponding adjacent normal tissues. *P<0.05 vs adjacent normal tissues. (F) The mRNA level of FGFR1 in OS cell lines (U2OS, MG-63, HOS, and SAOS-2) and normal human osteoblast cells (hFOB 1.19) was determined through RT-qPCR analysis. *P<0.05 vs hFOB1.19. (G) The expression correlation between miR-761 and FGFR1 mRNA in OS tissues was analyzed using Spearman’s correlation analysis. R2=0.3226, P<0.0001.

    Journal: OncoTargets and Therapy

    Article Title:

    MicroRNA-761 targets FGFR1 to suppress the malignancy of osteosarcoma by deactivating PI3K/Akt pathway

    doi: 10.2147/ott.s208263

    Figure Lengend Snippet: Figure 3 FGFR1 is a direct target gene of miR-761 in OS cells. (A) The wild-type and mutant miR-761 binding sites in the 3ʹ-UTR of FGFR1. (B) Agomir-NC or agomir-761 was co-transfected with pmirGLO-WT-FGFR1-3ʹ-UTR and pmirGLO-MUT-FGFR1-3ʹ-UTR into U2OS and HOS cells. After 48 h culture, luciferase reporter assay was carried out to explore whether the 3ʹ-UTR of FGFR1 could be directly targeted by miR-761. *P<0.05 vs agomir-NC. (C, D) The mRNA and protein levels of FGFR1 in U2OS and HOS cells after gain of miR-761 were examined through RT-qPCR and Western blot analysis. *P<0.05 vs agomir-NC. (E) RT-qPCR was utilized to quantify FGFR1 mRNA expression in 61 pairs of OS tissues and corresponding adjacent normal tissues. *P<0.05 vs adjacent normal tissues. (F) The mRNA level of FGFR1 in OS cell lines (U2OS, MG-63, HOS, and SAOS-2) and normal human osteoblast cells (hFOB 1.19) was determined through RT-qPCR analysis. *P<0.05 vs hFOB1.19. (G) The expression correlation between miR-761 and FGFR1 mRNA in OS tissues was analyzed using Spearman’s correlation analysis. R2=0.3226, P<0.0001.

    Article Snippet: Cell culture Normal human osteoblast cells (hFOB1.19) and four human OS cell lines (U2OS, MG-63, HOS and SAOS-2) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Mutagenesis, Binding Assay, Transfection, Luciferase, Reporter Assay, Quantitative RT-PCR, Western Blot, Expressing